Hotspot

pafI-trnS-GGA

LSC · 43935-44823 · 889 bp

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Pi 0.0107

MarkerSeek score 63.50

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 889
variable_sites: 22
pi: 0.0107
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1012-1023 bp	1.0	68.2
pafI-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1013-1024 bp	1.0	68.2
pafI-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1055-1066 bp	1.0	67.8
pafI-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1056-1067 bp	1.0	67.8
pafI-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	CCAATGCTACGCTTTCAACC	45.0	50.0	56.4	57.7	1015-1026 bp	1.0	67.6