Hotspot

psbK-psbI

LSC · 7940-8389 · 450 bp

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Pi 0.0229

MarkerSeek score 59.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 24
pi: 0.0229
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.9	58.1	789-822 bp	1.0	86.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TCGAATCCCTCTCTTTCCGT	50.0	50.0	57.9	58.2	716-749 bp	1.0	86.2
psbK-psbI_p3	TTCGTCAATTCCAGCCCAAA	ATTGGGAGAGATGGCTGAGT	45.0	50.0	58.0	58.1	1241-1288 bp	1.0	85.8
psbK-psbI_p4	TTCGTCAATTCCAGCCCAAA	TCGAATCCCTCTCTTTCCGT	45.0	50.0	58.0	58.2	1168-1215 bp	1.0	85.6
psbK-psbI_p5	TTCGTCAATTCCAGCCCAAA	CCTCTCTTTCCGTTTCCGTT	45.0	50.0	58.0	57.8	1161-1208 bp	1.0	85.5