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trnK-UUU-rps16

LSC · 4419-5252 · 834 bp

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Pi 0.0198

MarkerSeek score 77.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 36
pi: 0.0198
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	943-980 bp	1.0	82.2
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	942-979 bp	1.0	82.2
trnK-UUU-rps16_p3	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	958-995 bp	1.0	81.3
trnK-UUU-rps16_p4	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1028-1065 bp	1.0	81.2
trnK-UUU-rps16_p5	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1027-1064 bp	1.0	81.1