Hotspot

ndhD

SSC · 119802-121301 · 1500 bp

Back to Sinojackia

Pi 0.000578

MarkerSeek score 34.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 2
pi: 0.000578
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAACGAATAATTTCAAAAATCATAGCA	GCACGGGTTTTTCTGGTCTA	25.9	50.0	56.3	58.1	1602-1604 bp	1.0	40.2
ndhD_p2	ACAACGAATAATTTCAAAAATCATAGC	GCACGGGTTTTTCTGGTCTA	25.9	50.0	56.3	58.1	1603-1605 bp	1.0	40.2
ndhD_p3	ATTTCAAAAATCATAGCATAGGA	GCACGGGTTTTTCTGGTCTA	26.1	50.0	52.2	58.1	1593-1595 bp	1.0	40.2
ndhD_p4	TTTCAAAAATCATAGCATAGGAT	GCACGGGTTTTTCTGGTCTA	26.1	50.0	52.2	58.1	1592-1594 bp	1.0	40.2
ndhD_p5	CAACGAATAATTTCAAAAATCATAGCA	TTTCGAGCACGGGTTTTTCT	25.9	45.0	56.3	58.3	1608-1610 bp	1.0	40.2