Hotspot

ycf15-trnL-CAA

IRb · 97434-97803 · 370 bp

Back to Sinojackia

Pi 0.00649

MarkerSeek score 39.50

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 370
variable_sites: 4
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	TCTTGAACAGCGAACAACCA	AAATGGTAGACACGCGAGAC	45.0	50.0	58.0	58.0	555 bp	1.0	80.3
ycf15-trnL-CAA_p2	TCTTGAACAGCGAACAACCA	TGAAATGGTAGACACGCGAG	45.0	50.0	58.0	58.0	557 bp	1.0	80.3
ycf15-trnL-CAA_p3	TCTTGAACAGCGAACAACCA	GCCTTGGTGGTGAAATGGTA	45.0	50.0	58.0	58.1	567 bp	1.0	79.7
ycf15-trnL-CAA_p4	TCTTTCGGATAGGCCCTGAA	AAATGGTAGACACGCGAGAC	50.0	50.0	58.1	58.0	501 bp	1.0	79.6
ycf15-trnL-CAA_p5	TCTTTCGGATAGGCCCTGAA	TGAAATGGTAGACACGCGAG	50.0	50.0	58.1	58.0	503 bp	1.0	79.6