Hotspot

rpl20-rps12

LSC · 72716-73513 · 798 bp

Back to Sinojackia

Pi 0

MarkerSeek score 45.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACCTCGAAAGCTTGATGCAA	AATGTCACGAAATCTCCCGC	45.0	50.0	58.1	58.6	917-942 bp	1.0	76.3
rpl20-rps12_p2	AATGAGCACCTCGAAAGCTT	AATGTCACGAAATCTCCCGC	45.0	50.0	57.8	58.6	924-949 bp	1.0	75.6
rpl20-rps12_p3	ACCTCGAAAGCTTGATGCAA	CACGAAATCTCCCGCTCTTC	45.0	55.0	58.1	58.7	912-937 bp	1.0	75.6
rpl20-rps12_p4	ACCTCGAAAGCTTGATGCAA	CAGCGTCGAGGAACATGTAC	45.0	55.0	58.1	58.7	881-906 bp	1.0	75.6
rpl20-rps12_p5	TCCCCGTCTAATTCTGGTCA	AATGTCACGAAATCTCCCGC	50.0	50.0	57.8	58.6	860-885 bp	1.0	75.3