Hotspot

ndhC-trnM-CAU

LSC · 53183-55197 · 2015 bp

Back to Sinojackia

Pi 0.000926

MarkerSeek score 52.20

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 2015
variable_sites: 5
pi: 0.000926
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	GGGGCTAAAACTCCGGAAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	2157 bp	1.0	75.8
ndhC-trnM-CAU_p2	CTGGTCCTTTGCTAATCGGG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.3	58.4	2174 bp	1.0	74.2
ndhC-trnM-CAU_p3	CCTTTGCTAATCGGGGCTAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.7	58.4	2169 bp	1.0	74.2
ndhC-trnM-CAU_p4	GGGGCTAAAACTCCGGAAAT	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	2156 bp	1.0	73.8
ndhC-trnM-CAU_p5	GGGGCTAAAACTCCGGAAAT	TTTGAACCAATGACTCCCGC	50.0	50.0	57.9	58.8	2172 bp	1.0	73.0