Hotspot

atpA-atpF

LSC · 12567-12630 · 64 bp

Back to Sinojackia

Pi 0

MarkerSeek score 43.70

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ACTTGAAGTACGGTACCGGT	TGGTATGTTTGGAGCGATGA	50.0	45.0	58.4	56.9	204-207 bp	1.0	69.2
atpA-atpF_p2	TTGCTCAATACGTTCACGGA	TGGTATGTTTGGAGCGATGA	45.0	45.0	57.6	56.9	157-160 bp	1.0	68.9
atpA-atpF_p3	TCGCCTACTTGAAGTACGGT	TGGTATGTTTGGAGCGATGA	50.0	45.0	58.5	56.9	210-213 bp	1.0	68.5
atpA-atpF_p4	ACTAATTTCGTCGGCTCGAA	TGGTATGTTTGGAGCGATGA	45.0	45.0	57.4	56.9	130-133 bp	1.0	67.5
atpA-atpF_p5	ACTTGAAGTACGGTACCGGT	TGGTATGTTTGGAGCGATGAA	50.0	42.9	58.4	57.7	204-207 bp	1.0	67.0