Hotspot

trnN-GUU-ndhF

IRb · 96341-98877 · 2537 bp

Back to Sinogentiana

Pi 0.158

MarkerSeek score 85.50

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2537
variable_sites: 379
pi: 0.158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	CTTCATGTCTGGTTGCCTGA	50.0	50.0	58.2	57.8	2619-2767 bp	1.0	89.2
trnN-GUU-ndhF_p2	CCCCAAGTAGGATTCGAACC	CTTCATGTCTGGTTGCCTGA	55.0	50.0	57.7	57.8	2627-2775 bp	1.0	87.8
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	CGTTGCTAAATCGGCACAAT	50.0	45.0	58.2	57.5	2648-2792 bp	1.0	86.4
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	TTCCCCTTCATGTCTGGTTG	50.0	50.0	58.2	57.4	2624-2772 bp	1.0	86.0
trnN-GUU-ndhF_p5	GGATTCGAACCTACGACCAA	CTTCATGTCTGGTTGCCTGA	50.0	50.0	57.3	57.8	2618-2766 bp	1.0	85.1