Hotspot

trnF-GAA-trnV-UAC

LSC · 44297-45030 · 734 bp

Back to Sinogentiana

Pi 0.0463

MarkerSeek score 80.00

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 33
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	GGGATAGCTCAGCTGGTAGA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	837 bp	0.5	58.4
trnF-GAA-trnV-UAC_p2	GGATGCTACATTGGGACTGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	859 bp	0.5	58.2
trnF-GAA-trnV-UAC_p3	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	801 bp	0.5	58.0
trnF-GAA-trnV-UAC_p4	CTGGTAGAGCAGAGGACTGA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.9	57.8	825 bp	0.5	57.3
trnF-GAA-trnV-UAC_p5	GGGATAGCTCAGCTGGTAGA	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.0	57.5	836 bp	0.5	55.7