Hotspot

trnL-UAA-trnF-GAA

LSC · 43932-44223 · 292 bp

Back to Sinogentiana

Pi 0.0498

MarkerSeek score 76.00

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 292
variable_sites: 13
pi: 0.0498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	346-407 bp	1.0	82.9
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	382-443 bp	1.0	82.3
trnL-UAA-trnF-GAA_p3	GTCCCGTTCTGCATGTCAAT	TCTACCAGCTGAGCTATCCC	50.0	55.0	58.5	58.0	415-476 bp	1.0	81.9
trnL-UAA-trnF-GAA_p4	TCCCGTTCTGCATGTCAATG	TCTACCAGCTGAGCTATCCC	50.0	55.0	58.5	58.0	414-475 bp	1.0	81.9
trnL-UAA-trnF-GAA_p5	GTCCCGTTCTGCATGTCAAT	TTTGAACTGGTGACACGAGG	50.0	50.0	58.5	58.1	451-512 bp	1.0	81.4