Hotspot

trnY-GUA-trnE-UUC

LSC · 27540-27594 · 55 bp

Back to Sinogentiana

Pi 0.127

MarkerSeek score 79.20

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 7
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACTTATTGCCA	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.7	58.3	164-171 bp	1.0	78.8
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACTTATTGCC	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.7	58.3	165-172 bp	1.0	78.7
trnY-GUA-trnE-UUC_p3	CCAGCGTAGACTTATTGCCA	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.7	57.7	185-192 bp	1.0	78.2
trnY-GUA-trnE-UUC_p4	ACCAGCGTAGACTTATTGCC	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.7	57.7	186-193 bp	1.0	78.2
trnY-GUA-trnE-UUC_p5	CCAGCGTAGACTTATTGCCA	AAGTAGCCCCCATCGTCTAG	50.0	55.0	57.7	58.3	197-204 bp	1.0	78.0