Hotspot

trnS-GCU-trnG-UCC

LSC · 6088-6531 · 444 bp

Back to Sinogentiana

Pi 0.111

MarkerSeek score 80.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 444
variable_sites: 44
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	55.0	38.1	58.6	55.5	498-586 bp	1.0	57.6
trnS-GCU-trnG-UCC_p2	ATCCACTCAGCCATCTCTCC	GGGTTAGAAAAACGAATCACACT	55.0	39.1	58.6	57.3	509-597 bp	1.0	56.3
trnS-GCU-trnG-UCC_p3	ATCCACTCAGCCATCTCTCC	AACGAATCACACTTTTACCACT	55.0	36.4	58.6	56.2	499-587 bp	1.0	55.7
trnS-GCU-trnG-UCC_p4	ATCCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	55.0	45.0	58.6	54.0	484-572 bp	1.0	54.0
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	57.9	38.1	58.1	55.5	497-585 bp	1.0	53.5