Hotspot

ycf1

SSC · 121854-126989 · 5136 bp

Back to Sinocrassula

Pi 0.00292

MarkerSeek score 50.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5136
variable_sites: 15
pi: 0.00292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGGCCAATATTTTTCGCTA	GGAAATGTATCCTCCCCACT	40.0	50.0	55.8	56.3	303 bp	1.0	49.3
ycf1_p2	TACGGCCAATATTTTTCGCT	GGAAATGTATCCTCCCCACT	40.0	50.0	55.8	56.3	304 bp	1.0	49.3
ycf1_p3	ACGGCCAATATTTTTCGCTA	GGAAATGTATCCTCCCCACTT	40.0	47.6	55.8	57.0	303 bp	1.0	47.4
ycf1_p4	TACGGCCAATATTTTTCGCT	GGAAATGTATCCTCCCCACTT	40.0	47.6	55.8	57.0	304 bp	1.0	47.4
ycf1_p5	GATACGGCCAATATTTTTCGCT	GGAAATGTATCCTCCCCACT	40.9	50.0	57.5	56.3	306 bp	1.0	46.8