Hotspot

trnG-UCC

LSC · 36827-36897 · 71 bp

Back to Sinocrassula

Pi 0.141

MarkerSeek score 72.50

Primer available yes

Label trnG-UCC

Feature Metadata

label_name: trnG-UCC
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 10
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC_p1	TTTGCTTCTCCTGACGGTTG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.4	58.0	985-1226 bp	1.0	84.5
trnG-UCC_p2	TTTGCTTCTCCTGACGGTTG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.4	58.0	1321-1562 bp	1.0	82.9
trnG-UCC_p3	TTTGCTTCTCCTGACGGTTG	CAGGGGCAACAAGATCAAGT	50.0	50.0	58.4	58.1	1227-1468 bp	1.0	82.7
trnG-UCC_p4	TGGGCGTATCAAGAACGAAA	CGTTGTTTTTCGACCGGAAG	45.0	50.0	57.5	58.0	612 bp	0.5	56.2
trnG-UCC_p5	TGGGCGTATCAAGAACGAAA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.5	58.0	276 bp	0.5	56.0