Hotspot

rbcL

LSC · 54516-55943 · 1428 bp

Back to Sinocrassula

Pi 0

MarkerSeek score 32.70

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCGAGTAGACCTTGTTGTTGT	TGCGTCAATTAAGGCAAGGA	42.9	45.0	56.9	57.8	1520 bp	1.0	61.7
rbcL_p2	CGAGTAGACCTTGTTGTTGTG	TGCGTCAATTAAGGCAAGGA	47.6	45.0	56.9	57.8	1519 bp	1.0	61.4
rbcL_p3	CGCCTAATTCGAATTGATGTCG	TGCGTCAATTAAGGCAAGGA	45.5	45.0	58.2	57.8	1539 bp	1.0	60.8
rbcL_p4	GTCGAGTAGACCTTGTTGTTGT	TGCGTCAATTAAGGCAAGGA	45.5	45.0	58.3	57.8	1521 bp	1.0	60.2
rbcL_p5	CGAGTAGACCTTGTTGTTGTGA	TGCGTCAATTAAGGCAAGGA	45.5	45.0	58.3	57.8	1519 bp	1.0	60.2