Hotspot

psbH-petB

LSC · 73752-74466 · 715 bp

Back to Sinocrassula

Pi 0.0042

MarkerSeek score 61.20

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 3
pi: 0.0042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	AACGACTCCTTTGATGGGTG	CCGACAACTCTTCTTTCTGGT	50.0	47.6	57.8	57.9	874 bp	1.0	69.9
psbH-petB_p2	GATGGGGAACGACTCCTTTG	CCGACAACTCTTCTTTCTGGT	55.0	47.6	58.3	57.9	881 bp	1.0	69.3
psbH-petB_p3	GGAACGACTCCTTTGATGGG	CCGACAACTCTTCTTTCTGGT	55.0	47.6	58.3	57.9	876 bp	1.0	69.3
psbH-petB_p4	GGGAACGACTCCTTTGATGG	CCGACAACTCTTCTTTCTGGT	55.0	47.6	58.3	57.9	877 bp	1.0	69.3
psbH-petB_p5	GGGGAACGACTCCTTTGATG	CCGACAACTCTTCTTTCTGGT	55.0	47.6	58.3	57.9	878 bp	1.0	69.3