Pi
0.00976
MarkerSeek score
60.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GACCACCGCATTACAAATGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 478 bp | 1.0 | 79.9 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 460 bp | 1.0 | 79.6 |
| trnT-UGU-trnL-UAA_p3 | GACCACCGCATTACAAATGC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 486 bp | 1.0 | 79.4 |
| trnT-UGU-trnL-UAA_p4 | ATCCGTAAAAGCCGGCTATC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 512 bp | 1.0 | 79.3 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 468 bp | 1.0 | 79.1 |