Hotspot

psaA-ycf3

LSC · 42194-42786 · 593 bp

Back to Sinocrassula

Pi 0.00683

MarkerSeek score 63.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 4
pi: 0.00683
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	57.9	58.0	783-790 bp	1.0	80.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATAGCACTTACTCCCGGGAA	50.0	50.0	57.9	57.8	735-742 bp	1.0	78.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TAGCACTTACTCCCGGGAAT	50.0	50.0	57.9	57.8	734-741 bp	1.0	78.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	697-704 bp	1.0	78.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	760-767 bp	1.0	78.1