Hotspot

psbM-trnD-GUC

LSC · 29782-30825 · 1044 bp

Back to Sinocrassula

Pi 0.00484

MarkerSeek score 62.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1044
variable_sites: 5
pi: 0.00484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCAGTAGGAACTAGAATGA	TCAATTGGTAAGAGCACCGC	42.9	50.0	55.5	58.6	1158-1168 bp	1.0	48.6
psbM-trnD-GUC_p2	AAGGCAGTAGGAACTAGAATGA	TCAATTGGTAAGAGCACCGC	40.9	50.0	56.3	58.6	1159-1169 bp	1.0	46.8
psbM-trnD-GUC_p3	AGGCAGTAGGAACTAGAATGAA	TCAATTGGTAAGAGCACCGC	40.9	50.0	56.3	58.6	1158-1168 bp	1.0	46.8
psbM-trnD-GUC_p4	AAAGGCAGTAGGAACTAGAATGA	TCAATTGGTAAGAGCACCGC	39.1	50.0	57.0	58.6	1160-1170 bp	1.0	44.5
psbM-trnD-GUC_p5	AAGGCAGTAGGAACTAGAATGAA	TCAATTGGTAAGAGCACCGC	39.1	50.0	57.0	58.6	1159-1169 bp	1.0	44.5