Hotspot

rpoB-trnC-GCA

LSC · 26519-27628 · 1110 bp

Back to Sinocrassula

Pi 0.004

MarkerSeek score 58.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1110
variable_sites: 4
pi: 0.004
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCCGTCAAGCCATG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1167-1276 bp	1.0	82.1
rpoB-trnC-GCA_p2	GTTCTTCCGTCAAGCCATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1166-1275 bp	1.0	82.1
rpoB-trnC-GCA_p3	AGTTCTTCCGTCAAGCCATG	AGTCTTGTGTTGATCAGGCG	50.0	50.0	58.2	58.2	1191-1300 bp	1.0	81.2
rpoB-trnC-GCA_p4	AGTTCTTCCGTCAAGCCATG	GTCTTGTGTTGATCAGGCGA	50.0	50.0	58.2	58.2	1190-1299 bp	1.0	81.1
rpoB-trnC-GCA_p5	AGTTCTTCCGTCAAGCCATG	TCTTGTGTTGATCAGGCGAC	50.0	50.0	58.2	58.2	1189-1298 bp	1.0	81.1