Hotspot

ycf1

SSC · 122640-128123 · 5484 bp

Back to Sinocarum

Pi 0.0275

MarkerSeek score 61.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 383
pi: 0.0275
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGGTCATACCACCTTCAT	GCTGAGGACTTCGATGAGTG	50.0	55.0	58.0	58.1	1377 bp	0.625	65.4
ycf1_p2	TGGGGTCATACCACCTTCAT	CCCTTCCCTTTGGTTCTGTC	50.0	55.0	58.0	58.1	1133 bp	0.625	65.4
ycf1_p3	TGGGGTCATACCACCTTCAT	CCTTCCCTTTGGTTCTGTCC	50.0	55.0	58.0	58.1	1132 bp	0.625	65.4
ycf1_p4	TGGGGTCATACCACCTTCAT	CTTCCCTTTGGTTCTGTCCC	50.0	55.0	58.0	58.1	1131 bp	0.625	65.4
ycf1_p5	TGGGGTCATACCACCTTCAT	GAAACCTTGGCACAGATCCA	50.0	50.0	58.0	58.1	1240 bp	0.625	65.4