Hotspot

rps15-ycf1

SSC · 122211-122639 · 429 bp

Back to Sinocarum

Pi 0.0542

MarkerSeek score 78.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 429
variable_sites: 53
pi: 0.0542
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GACCCCTTGTTGTCTTCGTT	ATCGATCCCATCCGTTTGTC	50.0	50.0	58.0	57.8	966-1013 bp	0.5	65.0
rps15-ycf1_p2	GGTGCTGAGGACCCATATTG	ATCGATCCCATCCGTTTGTC	55.0	50.0	58.0	57.8	1326-1373 bp	0.5	64.5
rps15-ycf1_p3	ATGAGTCGAAGAACACCGTG	ATCGATCCCATCCGTTTGTC	50.0	50.0	57.9	57.8	1354-1401 bp	0.5	64.2
rps15-ycf1_p4	GGTCATTTCGTGTAGCTGGT	ATCGATCCCATCCGTTTGTC	50.0	50.0	57.9	57.8	1296-1343 bp	0.5	64.0
rps15-ycf1_p5	TAGCTGGTGCAGTCATAGGT	ATCGATCCCATCCGTTTGTC	50.0	50.0	58.1	57.8	1284-1331 bp	0.5	63.8