Hotspot

rpl32-trnL-UAG

SSC · 112377-112973 · 597 bp

Back to Sinocarum

Pi 0.0557
MarkerSeek score 84.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
597
variable_sites
81
pi
0.0557
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GTAAGGGATATTGGGCAGCC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 727-1032 bp 0.75 74.3
rpl32-trnL-UAG_p2 ATTGGGCAGCCTTAAAAGCT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 718-782 bp 0.625 67.7
rpl32-trnL-UAG_p3 ATTGGGCAGCCTTAAAAGCT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 719-783 bp 0.625 67.7
rpl32-trnL-UAG_p4 GGGCAGCCTTAAAAGCTTTG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 715-779 bp 0.625 67.4
rpl32-trnL-UAG_p5 GGGCAGCCTTAAAAGCTTTG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 716-780 bp 0.625 67.4