Hotspot

ndhF-rpl32

SSC · 111331-112211 · 881 bp

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Pi 0.0572

MarkerSeek score 83.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 881
variable_sites: 113
pi: 0.0572
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTGTGAACCAAAACCCTA	CAAAGCTTTTAAGGCTGCCC	50.0	50.0	58.0	57.9	1441-1759 bp	1.0	90.2
ndhF-rpl32_p2	GTTGCCGACGGAAAAAGAAG	AGCTTTTAAGGCTGCCCAAT	50.0	45.0	58.0	58.1	1072-1193 bp	0.375	61.2
ndhF-rpl32_p3	TGCCGACGGAAAAAGAAGAA	AGCTTTTAAGGCTGCCCAAT	45.0	45.0	58.0	58.1	1070-1191 bp	0.375	61.1
ndhF-rpl32_p4	TTGCCGACGGAAAAAGAAGA	AGCTTTTAAGGCTGCCCAAT	45.0	45.0	58.0	58.1	1071-1192 bp	0.375	61.1
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	AGCTTTTAAGGCTGCCCAAT	50.0	45.0	58.0	58.1	1478-1599 bp	0.375	60.7