Hotspot

petA-psbJ

LSC · 61400-62058 · 659 bp

Back to Sinocarum

Pi 0.0498

MarkerSeek score 80.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 85
pi: 0.0498
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTCGAGAAGGTTCA	CTGGATTGGGTTCATCTCTGT	40.9	47.6	57.6	57.4	726-1147 bp	1.0	58.3
petA-psbJ_p2	TCGAGAAGGTTCAATTGTCAGA	CTGGATTGGGTTCATCTCTGT	40.9	47.6	57.4	57.4	717-1138 bp	1.0	56.8
petA-psbJ_p3	AGAAACAGTTCGAGAAGGTTCA	TGGATTGGGTTCATCTCTGT	40.9	45.0	57.6	56.1	725-1146 bp	1.0	56.5
petA-psbJ_p4	AGAAACAGTTCGAGAAGGTTCA	TCTGGATTGGGTTCATCTCTG	40.9	47.6	57.6	57.1	727-1148 bp	1.0	56.1
petA-psbJ_p5	TCGAGAAGGTTCAATTGTCAGA	TGGATTGGGTTCATCTCTGT	40.9	45.0	57.4	56.1	716-1137 bp	1.0	54.9