Hotspot

psaI-ycf4

LSC · 58431-58812 · 382 bp

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Pi 0.041

MarkerSeek score 79.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 41
pi: 0.041
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATGGCAATGGCTTCTTT	GACCCCGTTATAGGTTGTATCC	45.0	50.0	57.9	57.7	483-512 bp	1.0	71.4
psaI-ycf4_p2	GCAATGGCAATGGCTTCTTT	ACCCCGTTATAGGTTGTATCCA	45.0	45.5	57.9	58.3	482-511 bp	1.0	70.8
psaI-ycf4_p3	GCAATGGCAATGGCTTCTTT	CCCCGTTATAGGTTGTATCCA	45.0	47.6	57.9	56.6	481-510 bp	1.0	70.3
psaI-ycf4_p4	GCAATGGCAATGGCTTCTTT	ACCCCGTTATAGGTTGTATCC	45.0	47.6	57.9	56.6	482-511 bp	1.0	70.2
psaI-ycf4_p5	GCAATGGCAATGGCTTCTTT	CCCCGTTATAGGTTGTATCCAT	45.0	45.5	57.9	57.1	481-510 bp	1.0	66.3