Hotspot

trnE-UUC-trnT-GGU

LSC · 30717-31461 · 745 bp

Back to Sinocarum

Pi 0.0496

MarkerSeek score 80.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 82
pi: 0.0496
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	646-814 bp	0.875	84.8
trnE-UUC-trnT-GGU_p2	CCGTTGCCTCCTTGAAAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.8	57.9	647-815 bp	0.875	79.5
trnE-UUC-trnT-GGU_p3	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	59.1	57.9	618-786 bp	0.875	77.4
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	ACCGCTGAGTTAAAAGGGC	50.0	52.6	57.8	58.1	645-813 bp	0.875	77.2
trnE-UUC-trnT-GGU_p5	CCGTTGCCTCCTTGAAAGA	TACCGCTGAGTTAAAAGGGC	52.6	50.0	57.7	57.9	647-815 bp	0.875	75.6