Hotspot

psbM-trnD-GUC

LSC · 29640-30260 · 621 bp

Back to Sinocarum

Pi 0.0422
MarkerSeek score 81.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
621
variable_sites
63
pi
0.0422
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AAATGCAAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 26.1 50.0 52.7 58.5 706-791 bp 1.0 48.9
psbM-trnD-GUC_p2 AATGCAAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 26.1 50.0 52.4 58.5 705-790 bp 1.0 48.9
psbM-trnD-GUC_p3 AAATGCAAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 25.0 50.0 53.3 58.5 706-791 bp 1.0 48.9
psbM-trnD-GUC_p4 AAATGCAAGAATATTTACTTCCA GTTCAATTGGTCAGAGCACC 26.1 50.0 52.7 57.0 707-792 bp 1.0 48.9
psbM-trnD-GUC_p5 AATGCAAGAATATTTACTTCCAT GTTCAATTGGTCAGAGCACC 26.1 50.0 52.4 57.0 706-791 bp 1.0 48.9