Hotspot

trnC-GCA-petN

LSC · 27836-28525 · 690 bp

Back to Sinocarum

Pi 0.0403

MarkerSeek score 85.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 70
pi: 0.0403
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	720-774 bp	1.0	89.7
trnC-GCA-petN_p2	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.9	58.2	760-814 bp	1.0	89.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	738-792 bp	1.0	87.3
trnC-GCA-petN_p4	GATATGGCCGAGTGGTAAGG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.5	58.2	759-813 bp	1.0	86.3
trnC-GCA-petN_p5	CGATATGGCCGAGTGGTAAG	GCCCAAGCGAGACTTACTAT	55.0	50.0	57.9	57.1	759-813 bp	1.0	83.5