Hotspot

atpF-atpH

LSC · 12398-12788 · 391 bp

Back to Sinocarum

Pi 0.044

MarkerSeek score 82.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 41
pi: 0.044
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	57.9	58.2	453-490 bp	1.0	88.6
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	454-491 bp	1.0	88.0
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	484-521 bp	1.0	87.7
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	TGGCCTGGTTGTAGCATTAG	45.0	50.0	57.9	57.6	455-492 bp	1.0	87.2
atpF-atpH_p5	AGTGGCCCAAAGAAACGAAA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	58.2	58.2	455-492 bp	1.0	87.2