Hotspot

trnK-UUU-rps16

LSC · 4100-4754 · 655 bp

Back to Sinocarum

Pi 0.0507
MarkerSeek score 84.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
655
variable_sites
83
pi
0.0507
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1509-1532 bp 0.75 77.5
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1508-1531 bp 0.75 77.5
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1508-1531 bp 0.75 77.5
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1507-1530 bp 0.75 77.5
trnK-UUU-rps16_p5 TCCCTCGCTTCATCCAAATG GATTCAAAGTGTATCGCGCG 50.0 50.0 58.0 58.0 1456-1479 bp 0.75 77.5