Hotspot

infA

LSC · 78995-79336 · 342 bp

Back to Sinobambusa

Pi 0.000975

MarkerSeek score 46.80

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 342
variable_sites: 1
pi: 0.000975
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	GTCTCTTCGCTTGAATTTTGGA	ATGGTAATCGCCCCTCCTAT	40.9	50.0	57.3	57.7	499-517 bp	1.0	58.2
infA_p2	AGTCTCTTCGCTTGAATTTTGG	ATGGTAATCGCCCCTCCTAT	40.9	50.0	57.2	57.7	500-518 bp	1.0	58.1
infA_p3	GTCTCTTCGCTTGAATTTTGGA	CGCCCCTCCTATAGTACTCG	40.9	60.0	57.3	58.5	491-509 bp	1.0	56.6
infA_p4	AGTCTCTTCGCTTGAATTTTGG	CGCCCCTCCTATAGTACTCG	40.9	60.0	57.2	58.5	492-510 bp	1.0	56.6
infA_p5	TCTCTTCGCTTGAATTTTGGAAC	ATGGTAATCGCCCCTCCTAT	39.1	50.0	57.9	57.7	498-516 bp	1.0	55.3