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psbM-petN

LSC · 18793-19546 · 754 bp

Back to Sinobambusa

Pi 0.000758

MarkerSeek score 43.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 2
pi: 0.000758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGGAAGTCAATATTCTCGCA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	54.5	58.2	941-960 bp	1.0	50.8
psbM-petN_p2	TGGAAGTCAATATTCTCGCA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	54.5	58.3	885-904 bp	1.0	49.8
psbM-petN_p3	TGGAAGTCAATATTCTCGCA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	54.5	58.4	884-903 bp	1.0	49.0
psbM-petN_p4	TGCTACTGCACTGTTTATTCT	TAGTAAGTCTCGCTTGGGCT	38.1	50.0	55.2	58.2	916-935 bp	1.0	48.9
psbM-petN_p5	TGCTACTGCACTGTTTATTCT	GTAGTATGGGGGAGGAGTGG	38.1	60.0	55.2	58.3	860-879 bp	1.0	47.9