Hotspot

trnG-UCC-trnT-GGU

LSC · 14453-16448 · 1996 bp

Back to Sinobambusa

Pi 0.000859
MarkerSeek score 46.50
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1996
variable_sites
5
pi
0.000859
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 38.1 52.4 55.5 58.1 2107-2131 bp 1.0 43.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT GACTTACGTCTTACCATGGCA 38.1 47.6 55.5 57.8 2120-2144 bp 1.0 42.9
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT TGACTTACGTCTTACCATGGC 38.1 47.6 55.5 57.8 2121-2145 bp 1.0 42.9
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2110-2134 bp 1.0 42.6
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2109-2133 bp 1.0 42.6