Hotspot

ndhH-trnN

SSC · 122517-128843 · 6327 bp

Back to Sinalliaria

Pi 0.0141

MarkerSeek score 48.50

Primer available yes

Label ndhH-trnN

Feature Metadata

label_name: ndhH-trnN
feature_type: igs
region: SSC
length_bp: 6327
variable_sites: 89
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-trnN_p1	TGAAAAACGAGTTGGCCTCA	TTTAACGGTTTGGGGACTGG	45.0	50.0	58.0	58.0	1211 bp	1.0	81.4
ndhH-trnN_p2	GCCCATGCAATCTGAGGTAA	TTTAACGGTTTGGGGACTGG	50.0	50.0	57.9	58.0	1065 bp	1.0	81.3
ndhH-trnN_p3	CTAGCCCATGCAATCTGAGG	TTTAACGGTTTGGGGACTGG	55.0	50.0	58.1	58.0	1068 bp	1.0	80.9
ndhH-trnN_p4	GCCGAATTTCCCCTATACCC	TTTAACGGTTTGGGGACTGG	55.0	50.0	57.8	58.0	1379 bp	1.0	80.2
ndhH-trnN_p5	TGAAAAACGAGTTGGCCTCA	TGGTTCTGCCAAAACAAAGC	45.0	45.0	58.0	57.7	1175 bp	1.0	79.1