Hotspot

rpl22-rps19

LSC · 83237-83293 · 57 bp

Back to Sinalliaria

Pi 0.0179

MarkerSeek score 64.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 1
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAAGAACGTCCACGGATT	TAAATTTTCGAGGACACGCG	50.0	45.0	58.0	56.9	221-222 bp	1.0	71.4
rpl22-rps19_p2	CGTAAGAACGTCCACGGATT	GAGGACACGCGAAAAATGAT	50.0	45.0	58.0	56.8	212-213 bp	1.0	71.2
rpl22-rps19_p3	CGTAAGAACGTCCACGGATT	TCGAGGACACGCGAAAAAT	50.0	47.4	58.0	57.8	214-215 bp	1.0	71.0
rpl22-rps19_p4	CGTAAGAACGTCCACGGATT	CGAGGACACGCGAAAAATGA	50.0	50.0	58.0	59.2	213-214 bp	1.0	70.9
rpl22-rps19_p5	CGTAAGAACGTCCACGGATT	TCGAGGACACGCGAAAAATG	50.0	50.0	58.0	59.2	214-215 bp	1.0	70.9