Hotspot

psaJ-rpl33

LSC · 66187-66631 · 445 bp

Back to Sinalliaria

Pi 0.0384

MarkerSeek score 67.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 17
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGTTCGTTTCGTTAGCAGGT	CGAACATCTTTACCTTTGGCC	50.0	47.6	58.5	57.5	545 bp	1.0	66.9
psaJ-rpl33_p2	GTTCGTTTCGTTAGCAGGTT	CGAACATCTTTACCTTTGGCC	45.0	47.6	56.7	57.5	544 bp	1.0	60.4
psaJ-rpl33_p3	GGTTCGTTTCGTTAGCAGGT	CTCGAACATCTTTACCTTTGGC	50.0	45.5	58.5	57.7	547 bp	1.0	60.1
psaJ-rpl33_p4	GGTTCGTTTCGTTAGCAGGT	TCGAACATCTTTACCTTTGGC	50.0	42.9	58.5	56.5	546 bp	1.0	59.1
psaJ-rpl33_p5	GGTTCGTTTCGTTAGCAGGT	GAACATCTTTACCTTTGGCCA	50.0	42.9	58.5	56.4	544 bp	1.0	58.2