Hotspot

petL-trnP

LSC · 64957-65647 · 691 bp

Back to Sinalliaria

Pi 0.0277

MarkerSeek score 65.60

Primer available yes

Label petL-trnP

Feature Metadata

label_name: petL-trnP
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 19
pi: 0.0277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-trnP_p1	TGGTCTAAGCAAAATACGACT	GGGTACAAAATGTCACGGGT	38.1	50.0	55.0	58.1	755-758 bp	1.0	49.4
petL-trnP_p2	TGGTCTAAGCAAAATACGACT	TGGGTACAAAATGTCACGGG	38.1	50.0	55.0	58.1	756-759 bp	1.0	49.4
petL-trnP_p3	TGGTCTAAGCAAAATACGACT	GTCACGGGTTCAAATCCTGT	38.1	50.0	55.0	58.1	744-747 bp	1.0	49.4
petL-trnP_p4	TGGTCTAAGCAAAATACGACT	CACGGGTTCAAATCCTGTCA	38.1	50.0	55.0	58.1	742-745 bp	1.0	49.4
petL-trnP_p5	TGGTCTAAGCAAAATACGACT	TGTCACGGGTTCAAATCCTG	38.1	50.0	55.0	58.1	745-748 bp	1.0	49.4