Hotspot

psbE-petL

LSC · 63492-64860 · 1369 bp

Back to Sinalliaria

Pi 0.028

MarkerSeek score 62.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1369
variable_sites: 38
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TCAGCCATTTATCCCGCTTC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2625-2665 bp	1.0	82.4
psbE-petL_p2	AAAGGATCAAAACGGCCTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2173-2188 bp	1.0	82.2
psbE-petL_p3	AAGGATCAAAACGGCCTGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2172-2187 bp	1.0	82.2
psbE-petL_p4	AAAGGATCAAAACGGCCTGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	2151-2166 bp	1.0	82.1
psbE-petL_p5	AAGGATCAAAACGGCCTGTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	2150-2165 bp	1.0	82.1