Hotspot

trnT-psbD

LSC · 30858-31948 · 1091 bp

Back to Sinalliaria

Pi 0.0101

MarkerSeek score 63.70

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 11
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	GTGGTAGAGTAACGCCATGG	CAAAAACAAAGCGGTCCCTC	55.0	50.0	58.1	57.9	1240-1252 bp	1.0	79.1
trnT-psbD_p2	TCAGTGGTAGAGTAACGCCA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.1	57.9	1243-1255 bp	1.0	78.9
trnT-psbD_p3	ACGGCCCTTTTAACTCAGTG	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.1	57.9	1257-1269 bp	1.0	78.8
trnT-psbD_p4	GACGGCCCTTTTAACTCAGT	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.8	57.9	1258-1270 bp	1.0	78.2
trnT-psbD_p5	GGCGTAAGTCATCGGTTCAA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.3	57.9	1217-1229 bp	1.0	77.4