Hotspot

psbM-trnD

LSC · 28227-29215 · 989 bp

Back to Sinalliaria

Pi 0.0144

MarkerSeek score 58.00

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 989
variable_sites: 14
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AGGAACGAGAATGAAGAGTGC	GTTCGAGCCCCGTCAGTC	47.6	66.7	58.0	60.1	1064-1082 bp	1.0	42.5
psbM-trnD_p2	ACGAGAATGAAGAGTGCAGT	GTTCGAGCCCCGTCAGTC	45.0	66.7	56.9	60.1	1060-1078 bp	1.0	42.5
psbM-trnD_p3	GGAACGAGAATGAAGAGTGCA	GTTCGAGCCCCGTCAGTC	47.6	66.7	58.3	60.1	1063-1081 bp	1.0	42.5
psbM-trnD_p4	AACGAGAATGAAGAGTGCAGT	GTTCGAGCCCCGTCAGTC	42.9	66.7	57.6	60.1	1061-1079 bp	1.0	42.5
psbM-trnD_p5	GGAACGAGAATGAAGAGTGC	GTTCGAGCCCCGTCAGTC	50.0	66.7	56.5	60.1	1063-1081 bp	1.0	42.5