Hotspot

trnR-atpA

LSC · 9120-9414 · 295 bp

Back to Sinalliaria

Pi 0.0556

MarkerSeek score 77.20

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 15
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	GCGAAAAGCGTCCATTGTC	TCAAGAGCAACTGGAACGTT	52.6	45.0	58.0	57.7	399-417 bp	1.0	76.3
trnR-atpA_p2	GCGAAAAGCGTCCATTGTC	TTCAAGAGCAACTGGAACGT	52.6	45.0	58.0	57.7	400-418 bp	1.0	76.3
trnR-atpA_p3	GCGAAAAGCGTCCATTGTC	ACATTAACCGCTGAAGCAGA	52.6	45.0	58.0	57.5	440-458 bp	1.0	74.9
trnR-atpA_p4	GCGAAAAGCGTCCATTGTC	AGACATTAACCGCTGAAGCA	52.6	45.0	58.0	57.5	442-460 bp	1.0	74.9
trnR-atpA_p5	GCGAAAAGCGTCCATTGTCT	TCAAGAGCAACTGGAACGTT	50.0	45.0	59.5	57.7	399-417 bp	1.0	72.5