Hotspot

trnS-trnG

LSC · 7614-8088 · 475 bp

Back to Sinalliaria

Pi 0.0347

MarkerSeek score 69.80

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 16
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	ACGCTTTAATCCACTCAGCC	AATCAAACCGAGAGACCCTT	50.0	45.0	58.3	56.5	580-587 bp	1.0	69.6
trnS-trnG_p2	ACGCTTTAATCCACTCAGCC	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.3	56.5	579-586 bp	1.0	69.6
trnS-trnG_p3	CGCTTTAATCCACTCAGCCA	AATCAAACCGAGAGACCCTT	50.0	45.0	58.3	56.5	579-586 bp	1.0	69.6
trnS-trnG_p4	CGCTTTAATCCACTCAGCCA	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.3	56.5	578-585 bp	1.0	69.6
trnS-trnG_p5	CGGAAGGGAAAGAGAGGGAT	ATCAAACCGAGAGACCCTTT	55.0	45.0	58.5	56.5	644-651 bp	1.0	67.3