Hotspot

ndhG

SSC · 124409-124945 · 537 bp

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Pi 0.482

MarkerSeek score 80.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 537
variable_sites: 227
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AAAAATTCCCGGTCGGTTCA	CAAGACACATCAGAGCACGA	45.0	50.0	58.0	57.9	977 bp	0.5	59.3
ndhG_p2	AAAAATTCCCGGTCGGTTCA	TCAAGACACATCAGAGCACG	45.0	50.0	58.0	57.9	978 bp	0.5	59.3
ndhG_p3	AAAATTCCCGGTCGGTTCAA	CAAGACACATCAGAGCACGA	45.0	50.0	58.0	57.9	976 bp	0.5	59.3
ndhG_p4	AAAATTCCCGGTCGGTTCAA	TCAAGACACATCAGAGCACG	45.0	50.0	58.0	57.9	977 bp	0.5	59.3
ndhG_p5	AAAATTCCCGGTCGGTTCAA	AGACACATCAGAGCACGAAC	45.0	50.0	58.0	57.9	974 bp	0.5	59.3