Hotspot

ndhA

SSC · 121196-123434 · 2239 bp

Back to Simarouba

Pi 0.485

MarkerSeek score 79.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2239
variable_sites: 933
pi: 0.485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGAGTTGAAGCCCCAAAAGG	TGCGCGTATTGTTTGTTGAC	50.0	45.0	58.0	58.0	2579 bp	0.5	59.8
ndhA_p2	AGTTGAAGCCCCAAAAGGAG	TGCGCGTATTGTTTGTTGAC	50.0	45.0	58.0	58.0	2577 bp	0.5	59.8
ndhA_p3	GAGTTGAAGCCCCAAAAGGA	TGCGCGTATTGTTTGTTGAC	50.0	45.0	58.0	58.0	2578 bp	0.5	59.8
ndhA_p4	GTTGAAGCCCCAAAAGGAGA	TGCGCGTATTGTTTGTTGAC	50.0	45.0	58.0	58.0	2576 bp	0.5	59.8
ndhA_p5	TCCCACTTTTGAATTGCCGA	TGCGCGTATTGTTTGTTGAC	45.0	45.0	58.0	58.0	2616 bp	0.5	59.7