Hotspot

ndhG-ndhE

SSC · 124946-125213 · 268 bp

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Pi 0.494

MarkerSeek score 75.40

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 268
variable_sites: 116
pi: 0.494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	AAAAATTCCCGGTCGGTTCA	CAAGACACATCAGAGCACGA	45.0	50.0	58.0	57.9	977 bp	0.5	59.3
ndhG-ndhE_p2	AAAAATTCCCGGTCGGTTCA	TCAAGACACATCAGAGCACG	45.0	50.0	58.0	57.9	978 bp	0.5	59.3
ndhG-ndhE_p3	AAAATTCCCGGTCGGTTCAA	CAAGACACATCAGAGCACGA	45.0	50.0	58.0	57.9	976 bp	0.5	59.3
ndhG-ndhE_p4	AAAATTCCCGGTCGGTTCAA	TCAAGACACATCAGAGCACG	45.0	50.0	58.0	57.9	977 bp	0.5	59.3
ndhG-ndhE_p5	AAAATTCCCGGTCGGTTCAA	AGACACATCAGAGCACGAAC	45.0	50.0	58.0	57.9	974 bp	0.5	59.3