Hotspot

ycf1-rps15

SSC · 119247-119635 · 389 bp

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Pi 0.484

MarkerSeek score 77.50

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 389
variable_sites: 167
pi: 0.484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	CGAGAAGCGGGTTATGTTGA	ACGATTAGGCGAAGAACACC	50.0	50.0	58.0	58.0	1281 bp	0.5	59.9
ycf1-rps15_p2	CGAGAAGCGGGTTATGTTGA	GATTAGGCGAAGAACACCGT	50.0	50.0	58.0	58.0	1279 bp	0.5	59.9
ycf1-rps15_p3	CGGACACCCTTTGGAGAAAA	ACGATTAGGCGAAGAACACC	50.0	50.0	58.0	58.0	1060 bp	0.5	59.8
ycf1-rps15_p4	CGGACACCCTTTGGAGAAAA	GATTAGGCGAAGAACACCGT	50.0	50.0	58.0	58.0	1058 bp	0.5	59.8
ycf1-rps15_p5	CGAGAAGCGGGTTATGTTGA	ATAAGCTAGCAGACGTTGGC	50.0	50.0	58.0	58.1	1013 bp	0.5	59.5