Hotspot

atpA-atpF

LSC · 13192-13250 · 59 bp

Back to Simarouba

Pi 0.0678

MarkerSeek score 74.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 4
pi: 0.0678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TACTTGAAGGACGGTACCGA	TGTTTGGGGCGATGAAAGAA	50.0	45.0	57.8	58.0	196-200 bp	1.0	79.9
atpA-atpF_p2	GCCTACTTGAAGGACGGTAC	TGTTTGGGGCGATGAAAGAA	55.0	45.0	57.7	58.0	199-203 bp	1.0	79.2
atpA-atpF_p3	TACTTGAAGGACGGTACCGA	ATGTTTGGGGCGATGAAAGA	50.0	45.0	57.8	57.8	197-201 bp	1.0	78.3
atpA-atpF_p4	ACTTGAAGGACGGTACCGAT	TGTTTGGGGCGATGAAAGAA	50.0	45.0	58.4	58.0	195-199 bp	1.0	78.0
atpA-atpF_p5	TACTTGAAGGACGGTACCGA	CATGTTTGGGGCGATGAAAG	50.0	50.0	57.8	57.7	198-202 bp	1.0	77.7