Hotspot

rps15

SSC · 113570-113809 · 240 bp

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Pi 0.558

MarkerSeek score 75.30

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 240
variable_sites: 130
pi: 0.558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GCAAGTAACCCAATGCACAC	GTAAATCAGCAGCGACTCCA	50.0	50.0	57.9	58.0	1169 bp	0.5	59.4
rps15_p2	GCAAGTAACCCAATGCACAC	TAAATCAGCAGCGACTCCAC	50.0	50.0	57.9	58.0	1168 bp	0.5	59.4
rps15_p3	GCAAGTAACCCAATGCACAC	TGAGGTAAATCAGCAGCGAC	50.0	50.0	57.9	58.0	1173 bp	0.5	59.4
rps15_p4	TTTCTGCTGTCCGTGATCTG	GTAAATCAGCAGCGACTCCA	50.0	50.0	57.9	58.0	1207 bp	0.5	59.2
rps15_p5	TTTCTGCTGTCCGTGATCTG	TAAATCAGCAGCGACTCCAC	50.0	50.0	57.9	58.0	1206 bp	0.5	59.2